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eclipse ti e c2 confocal laser scanning microscope  (Nikon)


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    Structured Review

    Nikon eclipse ti e c2 confocal laser scanning microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E C2 Confocal Laser Scanning Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti-e+microscope/C2%2B/pmc12811640-78-16-23
    Average 99 stars, based on 1 article reviews
    eclipse ti e c2 confocal laser scanning microscope - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7"

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    Journal: Biofilm

    doi: 10.1016/j.bioflm.2025.100335

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.
    Figure Legend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Techniques Used: Staining, Mutagenesis, Fluorescence, Microscopy

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    Article Title: Bactericidal activity of mammalian histones is caused by large membrane pore formation.
    Article Snippet: Phase contrast and fluorescence images were acquired using a Nikon Eclipse Ti-E microscope (Nikon, Melville, NY) containing a Nikon 40X and 100X Plan Apo (1.45 N.A.) objective, a 1.5X magnifier, a Sola light engine (Lumencor, Beaverton, OR), an LED-DA/FI/ TX filter set (Semrock, Rochester, NY) containing a 409/493/596 dichroic filter, a Hamamatsu Orca Flash 4.0 V2 camera (Hamamatsu, Bridgewater, NJ), and an Andor DU-897 EMCCD camera (Andor Technology, Belfast, Northern Ireland).

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    Microscopy:

    Article Title: Bactericidal activity of mammalian histones is caused by large membrane pore formation
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    Article Title: Bactericidal activity of mammalian histones is caused by large membrane pore formation.
    Article Snippet: Phase contrast and fluorescence images were acquired using a Nikon Eclipse Ti-E microscope (Nikon, Melville, NY) containing a Nikon 40X and 100X Plan Apo (1.45 N.A.) objective, a 1.5X magnifier, a Sola light engine (Lumencor, Beaverton, OR), an LED-DA/FI/ TX filter set (Semrock, Rochester, NY) containing a 409/493/596 dichroic filter, a Hamamatsu Orca Flash 4.0 V2 camera (Hamamatsu, Bridgewater, NJ), and an Andor DU-897 EMCCD camera (Andor Technology, Belfast, Northern Ireland).

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    Article Snippet: Immunofluorescence staining was analyzed on a Nikon Eclipse Ti-E microscope with NIS Elements Advanced Research software version 4.30 (Nikon).

    Article Title: Human plastins are novel cytoskeletal pH sensors with a reduced F-actin bundling capacity at basic pH.
    Article Snippet: Time-lapse images (started ~2 min post-mixing) were recorded using Nikon Eclipse Ti-E microscope equipped with a TIRF illumination module (with a 15-mW laser), a Nikon CFI Plan Apochromat λ 100× oil objective (NA: 1.45), a perfect focus system (Nikon Instruments, Melville, NY), and an iXon Ultra 897 EMCCD camera (Andor Technology, Belfast, UK).

    Article Title: TGFβ1 and RGD Cooperatively Regulate SMAD2/3-Mediated Oncogenic Effects in Prostate Cancer Cells in Bio-Orthogonally Constructed Hydrogels.
    Article Snippet: Cell viability was analyzed using a live/ dead viability assay kit (Thermo Fisher Scientific).

    Article Title: Proinflammatory transcriptomic and kinomic alterations in astrocytes derived from patients with familial Alzheimer's disease
    Article Snippet: Coverslips were mounted with Fluoromount-G (Southern Biotech, 0100-01) and imaged via a Nikon Eclipse Ti-E microscope.

    Article Title: Poly(ε-Caprolactone)/Sodium Bicarbonate/β-Tricalcium Phosphate Composites: Surface Characterization and Early Biological Response.
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    Staining:

    Article Title: Bactericidal activity of mammalian histones is caused by large membrane pore formation
    Article Snippet: Phase contrast and fluorescence images were acquired using a Nikon Eclipse Ti-E microscope (Nikon, Melville, NY) containing a Nikon 40X and 100X Plan Apo (1.45 N.A.) objective, a 1.5X magnifier, a Sola light engine (Lumencor, Beaverton, OR), an LED-DA/FI/TX filter set (Semrock, Rochester, NY) containing a 409/493/596 dichroic filter, a Hamamatsu Orca Flash 4.0 V2 camera (Hamamatsu, Bridgewater, NJ), and an Andor DU-897 EMCCD camera (Andor Technology, Belfast, Northern Ireland).

    Article Title: Bactericidal activity of mammalian histones is caused by large membrane pore formation.
    Article Snippet: Phase contrast and fluorescence images were acquired using a Nikon Eclipse Ti-E microscope (Nikon, Melville, NY) containing a Nikon 40X and 100X Plan Apo (1.45 N.A.) objective, a 1.5X magnifier, a Sola light engine (Lumencor, Beaverton, OR), an LED-DA/FI/ TX filter set (Semrock, Rochester, NY) containing a 409/493/596 dichroic filter, a Hamamatsu Orca Flash 4.0 V2 camera (Hamamatsu, Bridgewater, NJ), and an Andor DU-897 EMCCD camera (Andor Technology, Belfast, Northern Ireland).

    Article Title: Generation of the ICGi019-B-1 and ICGi019-B-2 lines via correction of the p.Met659Ile (c.1977G>A) variant in MYH7 of patient-specific induced pluripotent stem cells using CRISPR/Cas9
    Article Snippet: Immunofluorescence staining was analyzed on a Nikon Eclipse Ti-E microscope with NIS Elements Advanced Research software version 4.30 (Nikon).

    Article Title: Human plastins are novel cytoskeletal pH sensors with a reduced F-actin bundling capacity at basic pH.
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    Article Title: TGFβ1 and RGD Cooperatively Regulate SMAD2/3-Mediated Oncogenic Effects in Prostate Cancer Cells in Bio-Orthogonally Constructed Hydrogels.
    Article Snippet: Cell viability was analyzed using a live/ dead viability assay kit (Thermo Fisher Scientific).

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    Article Snippet: Coverslips were mounted with Fluoromount-G (Southern Biotech, 0100-01) and imaged via a Nikon Eclipse Ti-E microscope.

    Article Title: Poly(ε-Caprolactone)/Sodium Bicarbonate/β-Tricalcium Phosphate Composites: Surface Characterization and Early Biological Response.
    Article Snippet: Immunofluorescence microscopy images were acquired using a Nikon Eclipse Ti-E microscope with Nikon Plan 40×/0.75 and Nikon Plan 10×/0.10 objectives (Nikon Corporation, Tokyo, Japan).

    Article Title: Stm1 regulates Ifh1 activity revealing crosstalk between ribosome biogenesis and ribosome dormancy.
    Article Snippet: Live-cell microscopy was performed using a Nikon Eclipse Ti-E microscope equipped with a Hamamatsu cam- era, a motorized XY stage and a piezo Z drive and using Micro-Manager (v.1.4.23) software.

    Software:

    Article Title: Bactericidal activity of mammalian histones is caused by large membrane pore formation
    Article Snippet: Phase contrast and fluorescence images were acquired using a Nikon Eclipse Ti-E microscope (Nikon, Melville, NY) containing a Nikon 40X and 100X Plan Apo (1.45 N.A.) objective, a 1.5X magnifier, a Sola light engine (Lumencor, Beaverton, OR), an LED-DA/FI/TX filter set (Semrock, Rochester, NY) containing a 409/493/596 dichroic filter, a Hamamatsu Orca Flash 4.0 V2 camera (Hamamatsu, Bridgewater, NJ), and an Andor DU-897 EMCCD camera (Andor Technology, Belfast, Northern Ireland).

    Article Title: Bactericidal activity of mammalian histones is caused by large membrane pore formation.
    Article Snippet: Phase contrast and fluorescence images were acquired using a Nikon Eclipse Ti-E microscope (Nikon, Melville, NY) containing a Nikon 40X and 100X Plan Apo (1.45 N.A.) objective, a 1.5X magnifier, a Sola light engine (Lumencor, Beaverton, OR), an LED-DA/FI/ TX filter set (Semrock, Rochester, NY) containing a 409/493/596 dichroic filter, a Hamamatsu Orca Flash 4.0 V2 camera (Hamamatsu, Bridgewater, NJ), and an Andor DU-897 EMCCD camera (Andor Technology, Belfast, Northern Ireland).

    Article Title: Generation of the ICGi019-B-1 and ICGi019-B-2 lines via correction of the p.Met659Ile (c.1977G>A) variant in MYH7 of patient-specific induced pluripotent stem cells using CRISPR/Cas9
    Article Snippet: Immunofluorescence staining was analyzed on a Nikon Eclipse Ti-E microscope with NIS Elements Advanced Research software version 4.30 (Nikon).

    Article Title: Human plastins are novel cytoskeletal pH sensors with a reduced F-actin bundling capacity at basic pH.
    Article Snippet: Time-lapse images (started ~2 min post-mixing) were recorded using Nikon Eclipse Ti-E microscope equipped with a TIRF illumination module (with a 15-mW laser), a Nikon CFI Plan Apochromat λ 100× oil objective (NA: 1.45), a perfect focus system (Nikon Instruments, Melville, NY), and an iXon Ultra 897 EMCCD camera (Andor Technology, Belfast, UK).

    Article Title: TGFβ1 and RGD Cooperatively Regulate SMAD2/3-Mediated Oncogenic Effects in Prostate Cancer Cells in Bio-Orthogonally Constructed Hydrogels.
    Article Snippet: Cell viability was analyzed using a live/ dead viability assay kit (Thermo Fisher Scientific).

    Article Title: Proinflammatory transcriptomic and kinomic alterations in astrocytes derived from patients with familial Alzheimer's disease
    Article Snippet: Coverslips were mounted with Fluoromount-G (Southern Biotech, 0100-01) and imaged via a Nikon Eclipse Ti-E microscope.

    Article Title: Poly(ε-Caprolactone)/Sodium Bicarbonate/β-Tricalcium Phosphate Composites: Surface Characterization and Early Biological Response.
    Article Snippet: Immunofluorescence microscopy images were acquired using a Nikon Eclipse Ti-E microscope with Nikon Plan 40×/0.75 and Nikon Plan 10×/0.10 objectives (Nikon Corporation, Tokyo, Japan).

    Article Title: Stm1 regulates Ifh1 activity revealing crosstalk between ribosome biogenesis and ribosome dormancy.
    Article Snippet: Live-cell microscopy was performed using a Nikon Eclipse Ti-E microscope equipped with a Hamamatsu cam- era, a motorized XY stage and a piezo Z drive and using Micro-Manager (v.1.4.23) software.

    Fluorescence:

    Article Title: Bactericidal activity of mammalian histones is caused by large membrane pore formation
    Article Snippet: Phase contrast and fluorescence images were acquired using a Nikon Eclipse Ti-E microscope (Nikon, Melville, NY) containing a Nikon 40X and 100X Plan Apo (1.45 N.A.) objective, a 1.5X magnifier, a Sola light engine (Lumencor, Beaverton, OR), an LED-DA/FI/TX filter set (Semrock, Rochester, NY) containing a 409/493/596 dichroic filter, a Hamamatsu Orca Flash 4.0 V2 camera (Hamamatsu, Bridgewater, NJ), and an Andor DU-897 EMCCD camera (Andor Technology, Belfast, Northern Ireland).

    Article Title: Bactericidal activity of mammalian histones is caused by large membrane pore formation.
    Article Snippet: Phase contrast and fluorescence images were acquired using a Nikon Eclipse Ti-E microscope (Nikon, Melville, NY) containing a Nikon 40X and 100X Plan Apo (1.45 N.A.) objective, a 1.5X magnifier, a Sola light engine (Lumencor, Beaverton, OR), an LED-DA/FI/ TX filter set (Semrock, Rochester, NY) containing a 409/493/596 dichroic filter, a Hamamatsu Orca Flash 4.0 V2 camera (Hamamatsu, Bridgewater, NJ), and an Andor DU-897 EMCCD camera (Andor Technology, Belfast, Northern Ireland).

    Article Title: Generation of the ICGi019-B-1 and ICGi019-B-2 lines via correction of the p.Met659Ile (c.1977G>A) variant in MYH7 of patient-specific induced pluripotent stem cells using CRISPR/Cas9
    Article Snippet: Immunofluorescence staining was analyzed on a Nikon Eclipse Ti-E microscope with NIS Elements Advanced Research software version 4.30 (Nikon).

    Article Title: Human plastins are novel cytoskeletal pH sensors with a reduced F-actin bundling capacity at basic pH.
    Article Snippet: Time-lapse images (started ~2 min post-mixing) were recorded using Nikon Eclipse Ti-E microscope equipped with a TIRF illumination module (with a 15-mW laser), a Nikon CFI Plan Apochromat λ 100× oil objective (NA: 1.45), a perfect focus system (Nikon Instruments, Melville, NY), and an iXon Ultra 897 EMCCD camera (Andor Technology, Belfast, UK).

    Article Title: TGFβ1 and RGD Cooperatively Regulate SMAD2/3-Mediated Oncogenic Effects in Prostate Cancer Cells in Bio-Orthogonally Constructed Hydrogels.
    Article Snippet: Cell viability was analyzed using a live/ dead viability assay kit (Thermo Fisher Scientific).

    Article Title: Proinflammatory transcriptomic and kinomic alterations in astrocytes derived from patients with familial Alzheimer's disease
    Article Snippet: Coverslips were mounted with Fluoromount-G (Southern Biotech, 0100-01) and imaged via a Nikon Eclipse Ti-E microscope.

    Article Title: Poly(ε-Caprolactone)/Sodium Bicarbonate/β-Tricalcium Phosphate Composites: Surface Characterization and Early Biological Response.
    Article Snippet: Immunofluorescence microscopy images were acquired using a Nikon Eclipse Ti-E microscope with Nikon Plan 40×/0.75 and Nikon Plan 10×/0.10 objectives (Nikon Corporation, Tokyo, Japan).

    Article Title: Stm1 regulates Ifh1 activity revealing crosstalk between ribosome biogenesis and ribosome dormancy.
    Article Snippet: Live-cell microscopy was performed using a Nikon Eclipse Ti-E microscope equipped with a Hamamatsu cam- era, a motorized XY stage and a piezo Z drive and using Micro-Manager (v.1.4.23) software.

    Northern Blot:

    Article Title: Bactericidal activity of mammalian histones is caused by large membrane pore formation
    Article Snippet: Phase contrast and fluorescence images were acquired using a Nikon Eclipse Ti-E microscope (Nikon, Melville, NY) containing a Nikon 40X and 100X Plan Apo (1.45 N.A.) objective, a 1.5X magnifier, a Sola light engine (Lumencor, Beaverton, OR), an LED-DA/FI/TX filter set (Semrock, Rochester, NY) containing a 409/493/596 dichroic filter, a Hamamatsu Orca Flash 4.0 V2 camera (Hamamatsu, Bridgewater, NJ), and an Andor DU-897 EMCCD camera (Andor Technology, Belfast, Northern Ireland).

    Article Title: Bactericidal activity of mammalian histones is caused by large membrane pore formation.
    Article Snippet: Phase contrast and fluorescence images were acquired using a Nikon Eclipse Ti-E microscope (Nikon, Melville, NY) containing a Nikon 40X and 100X Plan Apo (1.45 N.A.) objective, a 1.5X magnifier, a Sola light engine (Lumencor, Beaverton, OR), an LED-DA/FI/ TX filter set (Semrock, Rochester, NY) containing a 409/493/596 dichroic filter, a Hamamatsu Orca Flash 4.0 V2 camera (Hamamatsu, Bridgewater, NJ), and an Andor DU-897 EMCCD camera (Andor Technology, Belfast, Northern Ireland).

    Article Title: Generation of the ICGi019-B-1 and ICGi019-B-2 lines via correction of the p.Met659Ile (c.1977G>A) variant in MYH7 of patient-specific induced pluripotent stem cells using CRISPR/Cas9
    Article Snippet: Immunofluorescence staining was analyzed on a Nikon Eclipse Ti-E microscope with NIS Elements Advanced Research software version 4.30 (Nikon).

    Article Title: Human plastins are novel cytoskeletal pH sensors with a reduced F-actin bundling capacity at basic pH.
    Article Snippet: Time-lapse images (started ~2 min post-mixing) were recorded using Nikon Eclipse Ti-E microscope equipped with a TIRF illumination module (with a 15-mW laser), a Nikon CFI Plan Apochromat λ 100× oil objective (NA: 1.45), a perfect focus system (Nikon Instruments, Melville, NY), and an iXon Ultra 897 EMCCD camera (Andor Technology, Belfast, UK).

    Article Title: TGFβ1 and RGD Cooperatively Regulate SMAD2/3-Mediated Oncogenic Effects in Prostate Cancer Cells in Bio-Orthogonally Constructed Hydrogels.
    Article Snippet: Cell viability was analyzed using a live/ dead viability assay kit (Thermo Fisher Scientific).

    Article Title: Proinflammatory transcriptomic and kinomic alterations in astrocytes derived from patients with familial Alzheimer's disease
    Article Snippet: Coverslips were mounted with Fluoromount-G (Southern Biotech, 0100-01) and imaged via a Nikon Eclipse Ti-E microscope.

    Article Title: Poly(ε-Caprolactone)/Sodium Bicarbonate/β-Tricalcium Phosphate Composites: Surface Characterization and Early Biological Response.
    Article Snippet: Immunofluorescence microscopy images were acquired using a Nikon Eclipse Ti-E microscope with Nikon Plan 40×/0.75 and Nikon Plan 10×/0.10 objectives (Nikon Corporation, Tokyo, Japan).

    Article Title: Stm1 regulates Ifh1 activity revealing crosstalk between ribosome biogenesis and ribosome dormancy.
    Article Snippet: Live-cell microscopy was performed using a Nikon Eclipse Ti-E microscope equipped with a Hamamatsu cam- era, a motorized XY stage and a piezo Z drive and using Micro-Manager (v.1.4.23) software.



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    Nikon eclipse ti e inverted fluorescent microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E Inverted Fluorescent Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti-e+microscope/Objectives/bio_rxiv__64898__2026__03__24__714019-126-21-20
    Average 99 stars, based on 1 article reviews
    eclipse ti e inverted fluorescent microscope - by Bioz Stars, 2026-10
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    Nikon inverted microscope nikon ti e eclipse
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Inverted Microscope Nikon Ti E Eclipse, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti-e+microscope/Objectives/bio_rxiv__64898__2026__03__20__710068-89-8-10
    Average 99 stars, based on 1 article reviews
    inverted microscope nikon ti e eclipse - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Nikon eclipse ti e inverted microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti-e+microscope/Objectives/pm41870162-202-6-5
    Average 99 stars, based on 1 article reviews
    eclipse ti e inverted microscope - by Bioz Stars, 2026-10
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    Nikon eclipse ti e c2 confocal microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti E C2 Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti-e+microscope/C2%2B/pm41865230-112-6-11
    Average 99 stars, based on 1 article reviews
    eclipse ti e c2 confocal microscope - by Bioz Stars, 2026-10
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    Nikon inverted eclipse ti e microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Inverted Eclipse Ti E Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+ti-e+microscope/Inverted+Microscopes/pm41863795-684-7-6
    Average 99 stars, based on 1 article reviews
    inverted eclipse ti e microscope - by Bioz Stars, 2026-10
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    Image Search Results


    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Eclipse Ti-E C2+ confocal laser scanning microscope (Nikon) equipped with CFI Plan Apo VC 20x/1.2 and CFI Plan Apo VC 60x/1.2 WI objective lenses.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy